Methods

Storage, Transportation, and Shipment

Overall, 20 0.2 ml aliquots are stored per subject from plasma, 20 for serum, 6 for buffy coat, and 6 for red blood cells, and 10 1 ml aliquots from urine collection. One mirror half of the aliquots is stored at the collection centres to facilitate local research, and the other mirror half is sent to IARC.

This biological material will be used for measurements of markers of metabolism, somatic and germline mutations, epigenetics, nutritional biomarkers, hormones, and metabonomics to investigate the etiology of breast cancer in this specific setting.